In order to decrease the influence caused by low bandwidth and high latency on Media Access Control (MAC) layer in Underwater Acoustic Sensor Network (UWASN), an Evolutionary Game Theory based MAC (EGT-MAC) protocol was proposed. In EGT-MAC, each sensor node adopted two strategies including spatial multiplexing and temporal multiplexing. With the replication kinetics equation, each strategy got an evolutionary stable strategy and reached stable equilibrium of evolution. In this way, it improved channel utilization rate and data transmission efficiency to achieve performance optimization for MAC protocol. The simulation results show that EGT-MAC can improve the network throughput as well as the transmission rate of data packet.